In situ assessment of beta-hexosaminidase activity
We have adapted two methods to evaluate the beta-hexosaminidase (HEX) enzymatic activity in cultured cells, based on the use of (i) the fluorogenic substrate 4-methylumbelliferyl-6-sulfo-2-acetamido-2- deoxy-beta-D-glucopyranoside (MU-GlcNAc-6-SO4) and (ii) the naphthol AS-BI-N-acetyl-beta-D-glucosa...
| Veröffentlicht in: | BioTechniques. - 1988. - 19(1995), 3 vom: 02. Sept., Seite 434-40 |
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| Format: | Aufsatz |
| Sprache: | English |
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1995
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Technical Report Journal Article Glucuronates Isoenzymes Naphthols naphthol AS-BI-N-acetyl-beta-glucuronide Hymecromone 3T5NG4Q468 4-methylumbelliferyl-6-sulfo-2-acetamido-2-deoxy-beta-glucopyranoside 93751-71-4 mehr... |