Use of vaccinia virus poly(A) polymerase for RNA 3'-end labeling with a chain-terminating nucleotide or a short 3' homopolymer tract
Conditions are described for the 3'-end labeling of RNA with 32P 3'-dATP (3'-deoxyadenosine-5'-triphosphate), a chain-terminating nucleotide, using the poly(A) polymerase (PAP) encoded by vaccinia virus. Reaction time, divalent cation species and concentration, and the requiremen...
| Veröffentlicht in: | BioTechniques. - 1988. - 19(1995), 3 vom: 02. Sept., Seite 416-20, 422-5 |
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| Format: | Aufsatz |
| Sprache: | English |
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1995
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Technical Report Cations, Divalent Deoxyadenine Nucleotides Deoxyadenosines Phosphorus Radioisotopes Manganese 42Z2K6ZL8P RNA 63231-63-0 mehr... |