Use of vaccinia virus poly(A) polymerase for RNA 3'-end labeling with a chain-terminating nucleotide or a short 3' homopolymer tract
Conditions are described for the 3'-end labeling of RNA with 32P 3'-dATP (3'-deoxyadenosine-5'-triphosphate), a chain-terminating nucleotide, using the poly(A) polymerase (PAP) encoded by vaccinia virus. Reaction time, divalent cation species and concentration, and the requiremen...
| Publié dans: | BioTechniques. - 1991. - 19(1995), 3 vom: 02. Sept., Seite 416-20, 422-5 |
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| Format: | Article |
| Langue: | English |
| Publié: |
1995
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| Accès à la collection: | BioTechniques |
| Sujets: | Research Support, Non-U.S. Gov't Technical Report Journal Article Cations, Divalent Deoxyadenine Nucleotides Deoxyadenosines Phosphorus Radioisotopes Manganese 42Z2K6ZL8P RNA plus... |