Primer-induced labeling of pea and field bean chromosomes in situ and in suspension

Primer-induced labeling of pea and field bean chromosomes in situ and in suspension

A protocol for primed in situ DNA labeling (PRINS) was optimized for pea (Pisum sativum L.) and field bean (Vicia faba L.) chromosomes attached to coverslips. Cloned DNA or synthetic oligonucleotides were used as probes for repetitive DNA sequences (rDNA, Fok-element) and different reaction conditio...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 19(1995), 3 vom: 02. Sept., Seite 402-4; 407-8
1. Verfasser: Macas, J (VerfasserIn)
Weitere Verfasser: Dolezel, J, Gualberti, G, Pich, U, Schubert, I, Lucretti, S
Format: Aufsatz
Sprache:English
Veröffentlicht: 1995
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Research Support, Non-U.S. Gov't Technical Report DNA Primers DNA Probes DNA, Plant Deoxyuracil Nucleotides Fluoresceins deoxyuridine triphosphate 1173-82-6 Fluorescein TPY09G7XIR