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20250127052039.0 |
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231221s1985 xx ||||| 00| ||jpn c |
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| 041 |
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|a jpn
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| 100 |
1 |
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|a Hayakawa, M
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Heterogeneity of a KU-2 cell line derived from human renal cell carcinoma--differential susceptibilities of its sublines to NK cell-mediated cytotoxicity
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|c 1985
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 31.10.1985
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|a Date Revised 15.11.2006
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Natural killer (NK) cell activity against 4 different sublines of a permanent cell line KU-2 derived from human renal cell carcinoma were measured by 51Cr-release assay and single cell binding assay. KU-2 and its sublines exhibited different susceptibilities of interferon (IFN)-augmented NK cell cytotoxicity. Moreover, 2 out of 4 sublines changed in susceptibility to NK cell cytotoxicity in a few months. Along with the direct assay for NK cell activity, frequency and kinetics of NK cell-mediated cytotoxicity against 3 sublines were studied by single cell binding assay where spontaneous killing of target cells was directly visualized by the reading of trypan blue uptake into target cells among effector-target cell conjugates. The frequency of killer cells among conjugates was dependent on the susceptibility of the sublines to the IFN-augmented NK cell cytotoxicity. These results suggested the following: KU-2 cell line was not homogeneous but composed of a heterogeneous population of cells and even in one subline derived from a clone, the cells with different susceptibilities to NK cell-mediated cytotoxicity developed spontaneously in ordinary culture conditions with the passage of a certain period of time
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| 650 |
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|a English Abstract
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| 650 |
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|a Journal Article
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| 650 |
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7 |
|a Interferon Type I
|2 NLM
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| 700 |
1 |
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|a Osawa, A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nagakura, K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kikuchi, H
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Hinyokika kiyo. Acta urologica Japonica
|d 1962
|g 31(1985), 5 vom: 01. Mai, Seite 763-8
|w (DE-627)NLM012631779
|x 0018-1994
|7 nnns
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| 773 |
1 |
8 |
|g volume:31
|g year:1985
|g number:5
|g day:01
|g month:05
|g pages:763-8
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|d 31
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|c 05
|h 763-8
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