Use of a chemically modified T7 DNA polymerase for manual and automated sequencing of supercoiled DNA

Use of a chemically modified T7 DNA polymerase for manual and automated sequencing of supercoiled DNA

Procedures are presented for reliable and accurate nucleotide sequence analysis using as template supercoiled DNA prepared by a modified rapid boiling minipreparation protocol. This method yields DNA templates suitable for sequencing within 1 h of bacterial harvest. We describe optimal reaction cond...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 6(1988), 5 vom: 30. Mai, Seite 460-9
1. Verfasser: Toneguzzo, F (VerfasserIn)
Weitere Verfasser: Glynn, S, Levi, E, Mjolsness, S, Hayday, A
Format: Aufsatz
Sprache:English
Veröffentlicht: 1988
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't DNA, Superhelical DNA-Directed DNA Polymerase EC 2.7.7.7