Radioautographic analysis of surface markers on decidual cells shared by cells of the lymphomyeloid tissues
Stromal type decidual cells recovered from the murine decidua by a mild collagenase dispersion procedure contain immunoregulatory cells whose ultimate precursors may originate from the bone marrow. To explore the familial relationship of these cells with other cells of the immune system, a battery o...
| Veröffentlicht in: | American journal of reproductive immunology and microbiology : AJRIM. - 1985. - 9(1985), 2 vom: 15. Okt., Seite 39-47 |
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| Format: | Aufsatz |
| Sprache: | English |
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1985
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| Zugriff auf das übergeordnete Werk: | American journal of reproductive immunology and microbiology : AJRIM |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Ly Antigens, Surface Histocompatibility Antigens Class II Thy-1 Antigens |
| Zusammenfassung: | Stromal type decidual cells recovered from the murine decidua by a mild collagenase dispersion procedure contain immunoregulatory cells whose ultimate precursors may originate from the bone marrow. To explore the familial relationship of these cells with other cells of the immune system, a battery of cell surface markers recognized on lymphomyeloid cells were examined and quantitated at the morphological level on typical stromal type decidual cells of the dispersed CBA mouse decidua at 8-14 days of syngeneic pregnancy, using a sensitive radioautographic technique. Cells were either labeled directly by exposure to 125I-labeled monoclonal antibodies against Thy-1, Mac-1, or Lyt antigens or indirectly by a sequential exposure to monoclonal anti-I-Ak (Ia.17) or monospecific anti-I-JK antibodies and 125I-labeled Protein A. Decidual cells were found to be Thy-1+/- (13-73% positive, the incidence rising with advancing gestation in the decidua basalis), Mac-1+/- (present of 6-11% on day 8 and 17-32% on day 12), I-A-, 1-J-, and Lyt-. Macrophages within the decidua were Thy-1-, Lyt-, Mac-1+, and I-A+/- (present on 5-61% of cells, the incidence rising with advancing gestation).(ABSTRACT TRUNCATED AT 250 WORDS) |
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| Beschreibung: | Date Completed 19.12.1985 Date Revised 29.10.2019 published: Print Citation Status MEDLINE |
| ISSN: | 8755-8920 |