A spectrophotometric method for calmodulin assay

The activity of calmodulin as an activator of cAMP phosphodiesterase was assayed. AMP was hydrolyzed by 5'-nucleotidase, and the adenosine formed was measured by both liquid scintillation counting and spectrophotometry at 265 nm. Calmodulin activities measured by the two methods were equivalent...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 6(1988), 4 vom: 01. Apr., Seite 294-6
1. Verfasser: Garg, U C (VerfasserIn)
Weitere Verfasser: Rai, N, Singh, Y, Dhaunsi, G S, Sidhu, G S, Ganguly, N K, Bhatnagar, R
Format: Aufsatz
Sprache:English
Veröffentlicht: 1988
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Journal Article Calmodulin Cyclic AMP E0399OZS9N 3',5'-Cyclic-AMP Phosphodiesterases EC 3.1.4.17