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NLM028375157 |
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20250126072224.0 |
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tu |
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231221s1988 xx ||||| 00| ||eng c |
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|a pubmed25n0095.xml
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|a (DE-627)NLM028375157
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|a (NLM)2856199
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Verma, V
|e verfasserin
|4 aut
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| 245 |
1 |
3 |
|a An economical large-scale procedure to purify Micrococcus plasmid DNA
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| 264 |
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1 |
|c 1988
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 14.05.1990
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| 500 |
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|a Date Revised 21.11.2013
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| 500 |
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a A reproducible and economical procedure for obtaining a large yield of highly purified covalently closed circular (ccc) plasmid DNA from an industrially important strain of Micrococcus is described. The procedure adopted here departs in several ways from commonly used protocols for isolation of plasmid DNA from Gram (positive) and Gram (negative) bacteria. The plasmid DNA prepared by this procedure is free of contaminants and is pure enough to be used for electron microscopy, DNA transformation, sequencing, in vitro transcription and mutagenesis
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a Chlorides
|2 NLM
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| 650 |
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7 |
|a DNA, Bacterial
|2 NLM
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| 650 |
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7 |
|a DNA, Circular
|2 NLM
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| 650 |
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|a Sodium Dodecyl Sulfate
|2 NLM
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| 650 |
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7 |
|a 368GB5141J
|2 NLM
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| 650 |
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7 |
|a Lithium
|2 NLM
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| 650 |
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7 |
|a 9FN79X2M3F
|2 NLM
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| 650 |
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7 |
|a Ribonucleases
|2 NLM
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| 650 |
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7 |
|a EC 3.1.-
|2 NLM
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| 650 |
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7 |
|a Serine Endopeptidases
|2 NLM
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| 650 |
|
7 |
|a EC 3.4.21.-
|2 NLM
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| 650 |
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7 |
|a Endopeptidase K
|2 NLM
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| 650 |
|
7 |
|a EC 3.4.21.64
|2 NLM
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| 650 |
|
7 |
|a Lithium Chloride
|2 NLM
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| 650 |
|
7 |
|a G4962QA067
|2 NLM
|
| 700 |
1 |
|
|a Qazi, G N
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Parshad, R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chopra, C L
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 6(1988), 10 vom: 01. Nov., Seite 936, 938, 940
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:6
|g year:1988
|g number:10
|g day:01
|g month:11
|g pages:936, 938, 940
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|d 6
|j 1988
|e 10
|b 01
|c 11
|h 936, 938, 940
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