A system for on-line detection and resolution of radiolabeled DNA molecules and its application to automated DNA sequence analysis

We describe a system for the real-time detection of radioactively labeled DNA molecules in gel matrix, and we demonstrate the application of this system to DNA sequence analysis. DNA sequencing reactions prepared by the Sanger chain termination method are resolved by electrophoresis on 8% polyacryla...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 7(1989), 8 vom: 14. Sept., Seite 866-77
Auteur principal: Toneguzzo, F (Auteur)
Autres auteurs: Beck, J, Cahill, P, Ciarkowski, M, Page, G, Glynn, S, Hungerman, E, Levi, E, Ikeda, R, McKenney, K
Format: Article
Langue:English
Publié: 1989
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't DNA, Single-Stranded DNA, Superhelical Phosphorus Radioisotopes DNA 9007-49-2