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20250126030220.0 |
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tu |
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231221s1989 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed25n0090.xml
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|a (NLM)2698650
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Prickett, K S
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A calcium-dependent antibody for identification and purification of recombinant proteins
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| 264 |
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1 |
|c 1989
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
|
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
|
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|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
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|a Date Completed 24.05.1990
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| 500 |
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|a Date Revised 21.11.2013
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a We report a straightforward methodology for purification of recombinant proteins by incorporating a short hydrophilic peptide marker segment at their N-termini. A calcium-dependent antibody that reacts primarily with the first three amino acids of this peptide segment was used to affinity purify the fusion proteins in a single chromatographic step. The marker peptide could subsequently be removed by proteolysis with the enzyme enterokinase
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a Antibodies, Monoclonal
|2 NLM
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| 650 |
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7 |
|a Interleukin-3
|2 NLM
|
| 650 |
|
7 |
|a Recombinant Proteins
|2 NLM
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| 650 |
|
7 |
|a Interleukin-4
|2 NLM
|
| 650 |
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7 |
|a 207137-56-2
|2 NLM
|
| 650 |
|
7 |
|a Enteropeptidase
|2 NLM
|
| 650 |
|
7 |
|a EC 3.4.21.9
|2 NLM
|
| 650 |
|
7 |
|a Calcium
|2 NLM
|
| 650 |
|
7 |
|a SY7Q814VUP
|2 NLM
|
| 700 |
1 |
|
|a Amberg, D C
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hopp, T P
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 7(1989), 6 vom: 14. Juni, Seite 580-9
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
|
| 773 |
1 |
8 |
|g volume:7
|g year:1989
|g number:6
|g day:14
|g month:06
|g pages:580-9
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|d 7
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|h 580-9
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