Enhanced specificity in immunoscreening of expression cDNA clones using radiolabeled antigen overlay
A highly sensitive and specific method has been developed for immunoscreening clones from an expression cDNA library. The procedures utilize a radiolabeled antigen detection method described originally for the immunoblotting of plasma proteins (5). Screening of rat alpha 1-antitrypsin clones was use...
| Publié dans: | BioTechniques. - 1991. - 7(1989), 1 vom: 08. Jan., Seite 68-72 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
1989
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Antigens Iodine Radioisotopes alpha 1-Antitrypsin DNA 9007-49-2 |