Rapid one-step characterization of recombinant vectors by direct analysis of transformed Escherichia coli colonies
We have developed a polymerase chain reaction (PCR) based procedure for rapidly analyzing recombinant vectors in whole bacterial cells. No purification, restriction mapping or sequencing of vectors is required and the results are available within 6 hours. Whole cells are added to a PCR mix that is d...
| Publié dans: | BioTechniques. - 1991. - 7(1989), 7 vom: 07. Juli, Seite 689-90 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
1989
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article DNA, Bacterial Factor VIII 9001-27-8 DNA 9007-49-2 |