Targeted DNA sequencing : rapid identification of DNA clones by sequencing DNA using mixed oligodeoxynucleotide probes as primers
A rapid identification method involving targeted DNA sequencing of genomic or cDNA clones using mixed (degenerate) probes as primers is described. The strategy involves the use of the same mixed probes for sequencing the clone of interest as they are used for screening the DNA libraries. Probes cont...
| Veröffentlicht in: | BioTechniques. - 1988. - 9(1990), 1 vom: 15. Juli, Seite 28-32 |
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| Format: | Aufsatz |
| Sprache: | English |
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1990
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, U.S. Gov't, P.H.S. DNA, Recombinant DNA, Viral Oligonucleotide Probes DNA 9007-49-2 bacteriophage T7 induced DNA polymerase EC 2.7.7.- DNA-Directed DNA Polymerase |