Gene splicing by overlap extension : tailor-made genes using the polymerase chain reaction

Gene Splicing by Overlap Extension or "gene SOEing" is a PCR-based method of recombining DNA sequences without reliance on restriction sites and of directly generating mutated DNA fragments in vitro. By modifying the sequences incorporated into the 5'-ends of the primers, any pair of...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	BioTechniques. - 1988. - 8(1990), 5 vom: 30. Mai, Seite 528-35
1. Verfasser:	Horton, R M (VerfasserIn)
Weitere Verfasser:	Cai, Z L, Ho, S N, Pease, L R
Format:	Aufsatz
Sprache:	English
Veröffentlicht:	1990
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't DNA, Recombinant