Purification of a thermostable DNA polymerase from Thermus thermophilus HB8, useful in the polymerase chain reaction

A thermostable DNA polymerase, isolated from the thermophilic strain Thermus thermophilus HB 8 was purified by a five-step procedure which provides a high yield and a homogeneous preparation. The molecular weight was estimated to be 67,000 daltons and the extension rate was determined to be 1500 nuc...

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Veröffentlicht in:BioTechniques. - 1991. - 9(1990), 3 vom: 01. Sept., Seite 276-81
1. Verfasser: Carballeira, N (VerfasserIn)
Weitere Verfasser: Nazabal, M, Brito, J, Garcia, O
Format: Aufsatz
Sprache:English
Veröffentlicht: 1990
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article DNA 9007-49-2 DNA-Directed DNA Polymerase EC 2.7.7.7