High efficiency transient expression of eukaryotic genes : use of an HSV-1 immediate early promoter (ICP4)
We have compared the transcriptional efficiencies of a number of eukaryotic promoters following DNA-mediated transfection into cultured rat hepatoma cells. We find that the highest levels of expression for the bacterial chloramphenicol acetyltransferase (CAT) reporter gene are observed with a herpes...
| Veröffentlicht in: | BioTechniques. - 1991. - 9(1990), 2 vom: 01. Aug., Seite 168-73 |
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| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
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1990
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Immediate-Early Proteins Phosphoproteins Trans-Activators Viral Regulatory and Accessory Proteins herpes simplex virus, type 1 protein ICP4 Chloramphenicol O-Acetyltransferase |