PCR-mediated chemical mutagenesis of cloned duplex DNAs
We describe an efficient, PCR-mediated protocol for random chemical mutagenesis of cloned duplex DNAs. The method involves a single molecular cloning step and is compatible with a wide variety of recombinant DNA vectors. To illustrate the procedure, we report the nitrous acid mutagenesis of a human...
| Veröffentlicht in: | BioTechniques. - 1991. - 11(1991), 2 vom: 01. Aug., Seite 204-6, 208, 210-1 |
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| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1991
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Ribosomal Proteins ribosomal protein S14 DNA 9007-49-2 Nitrous Acid T2I5UM75DN |