Measurement of prostaglandin G/H synthase and lipoxygenase activity in the stomach wall by HPLC
A method for the determination of prostaglandin G/H synthase and lipoxygenase activities in tissues was developed and employed with rat gastric mucosa samples. Tissues and microsomes were incubated in a buffer containing nonionic detergent and 1.32 mM arachidonic acid for 10 min. Following extractio...
| Publié dans: | BioTechniques. - 1991. - 10(1991), 2 vom: 28. Feb., Seite 222-6 |
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| Auteur principal: | |
| Autres auteurs: | , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1991
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Arachidonic Acids Leukotrienes Arachidonic Acid 27YG812J1I Lipoxygenase EC 1.13.11.12 Prostaglandin-Endoperoxide Synthases EC 1.14.99.1 |