Measurement of prostaglandin G/H synthase and lipoxygenase activity in the stomach wall by HPLC
A method for the determination of prostaglandin G/H synthase and lipoxygenase activities in tissues was developed and employed with rat gastric mucosa samples. Tissues and microsomes were incubated in a buffer containing nonionic detergent and 1.32 mM arachidonic acid for 10 min. Following extractio...
Veröffentlicht in: | BioTechniques. - 1988. - 10(1991), 2 vom: 28. Feb., Seite 222-6 |
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1. Verfasser: | |
Weitere Verfasser: | , , |
Format: | Aufsatz |
Sprache: | English |
Veröffentlicht: |
1991
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Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Journal Article Arachidonic Acids Leukotrienes Arachidonic Acid 27YG812J1I Lipoxygenase EC 1.13.11.12 Prostaglandin-Endoperoxide Synthases EC 1.14.99.1 |