Measurement of prostaglandin G/H synthase and lipoxygenase activity in the stomach wall by HPLC

A method for the determination of prostaglandin G/H synthase and lipoxygenase activities in tissues was developed and employed with rat gastric mucosa samples. Tissues and microsomes were incubated in a buffer containing nonionic detergent and 1.32 mM arachidonic acid for 10 min. Following extractio...

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Veröffentlicht in:BioTechniques. - 1988. - 10(1991), 2 vom: 28. Feb., Seite 222-6
1. Verfasser: Stein, T A (VerfasserIn)
Weitere Verfasser: Bailey, B, Auguste, L J, Wise, L
Format: Aufsatz
Sprache:English
Veröffentlicht: 1991
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Arachidonic Acids Leukotrienes Arachidonic Acid 27YG812J1I Lipoxygenase EC 1.13.11.12 Prostaglandin-Endoperoxide Synthases EC 1.14.99.1