S1 nuclease transcript mapping using sequenase-derived single-stranded probes
We report here a simple method for the production of high specific activity single-stranded DNA probes for S1 nuclease transcript mapping. Plasmid DNA is used as in vitro DNA templates for probe production by primer extension using Sequenase. This allows the production of long single-stranded probes...
| Veröffentlicht in: | BioTechniques. - 1988. - 10(1991), 4 vom: 15. Apr., Seite 426, 428 |
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| Format: | Aufsatz |
| Sprache: | English |
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1991
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article DNA Probes DNA, Single-Stranded Methyltransferases EC 2.1.1.- bacteriophage T7 induced DNA polymerase EC 2.7.7.- DNA-Directed DNA Polymerase EC 2.7.7.7 Single-Strand Specific DNA and RNA Endonucleases |