Use of the polymerase chain reaction for screening and evaluation of recombinant baculovirus clones

We report the application of the PCR for screening and high-resolution characterization of recombinant baculovirus clones. Starting with less than 10 nanograms of viral DNA, it is possible to 1) demonstrate that the DNA sequence to be expressed has not been deleted or rearranged during the co-transf...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 11(1991), 4 vom: 01. Okt., Seite 512-9
1. Verfasser: Webb, A C (VerfasserIn)
Weitere Verfasser: Bradley, M K, Phelan, S A, Wu, J Q, Gehrke, L
Format: Aufsatz
Sprache:English
Veröffentlicht: 1991
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. DNA, Recombinant DNA, Viral