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|a eng
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| 100 |
1 |
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|a Wang, R F
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Comparison and modification of rRNA sequencing methods
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|c 1991
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 08.04.1992
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|a Date Revised 15.11.2006
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a In this study, modification of two methods of RNA sequencing resulted in more definitive sequencing bands. In one method of sequencing, the bands of lane A and lane G sometimes were not clear. Modifications of this method by changing the concentrations of ddATP and ddGTP resulted in the bands of lane A and lane G becoming more readable. Although a second sequencing method was found to have clearer bands than the first method, and the bases immediately downstream from the primer binding site could be read by using r-32P-labeled primer, the bands on the top of lane A still were not clear. Modifications of this second method by changing the ddATP/dATP ratio resulted in the bands of lane A becoming much clearer
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| 650 |
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4 |
|a Comparative Study
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| 650 |
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a RNA, Ribosomal
|2 NLM
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| 650 |
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|a RNA, Ribosomal, 16S
|2 NLM
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| 650 |
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7 |
|a DNA
|2 NLM
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| 650 |
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7 |
|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Cao, W W
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Slavik, M F
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1991
|g 11(1991), 4 vom: 01. Okt., Seite 438, 440
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
1 |
8 |
|g volume:11
|g year:1991
|g number:4
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|g month:10
|g pages:438, 440
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|d 11
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