Rapid 16S ribosomal DNA sequencing from a single colony without DNA extraction or purification
Ribosomal RNA sequences are useful for establishing phylogenetic relationships, for oligonucleotide probes and for characterization of uncultured organisms. We describe rapid ribosomal DNA sequencing using PCR with transcript sequencing. Nucleic acid specificity at three steps (amplification, transc...
| Veröffentlicht in: | BioTechniques. - 1991. - 11(1991), 1 vom: 01. Juli, Seite 40-4 |
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| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
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1991
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, U.S. Gov't, Non-P.H.S. DNA, Bacterial DNA, Ribosomal Deoxyribonucleotides RNA, Ribosomal, 16S RNA-Directed DNA Polymerase EC 2.7.7.49 |