Detection of bacterial mRNA using polymerase chain reaction
A method was developed for the detection of bacterial mRNAs using reverse transcriptase followed by the polymerase chain reaction (PCR) and Southern blot analysis. The method involves brief inhibition of protein synthesis with chloramphenicol, followed by reverse transcription, PCR amplification of...
Veröffentlicht in: | BioTechniques. - 1988. - 10(1991), 1 vom: 19. Jan., Seite 48-9 |
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1. Verfasser: | |
Weitere Verfasser: | , , , , |
Format: | Aufsatz |
Sprache: | English |
Veröffentlicht: |
1991
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Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Journal Article RNA, Bacterial RNA, Messenger Chloramphenicol 66974FR9Q1 RNA-Directed DNA Polymerase EC 2.7.7.49 |