A study of cytokeratin in human normal bladder epithelium and bladder carcinoma cell lines
Normal epithelial and carcinoma cells of human bladder were investigated for the cytokeratin which is one of the intermediate filaments and comprises cytoskeleton using the immunofluorescence method. Carcinoma cell lines used were JTC-30, JTC-32, HUB-41 and T-24. In normal urothelium, keratin fibers...
| Publié dans: | Hinyokika kiyo. Acta urologica Japonica. - 1962. - 36(1990), 8 vom: 29. Aug., Seite 903-6; Discussion 906-7 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article |
| Langue: | Japanese |
| Publié: |
1990
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| Accès à la collection: | Hinyokika kiyo. Acta urologica Japonica |
| Sujets: | English Abstract Journal Article Keratins 68238-35-7 |
| Résumé: | Normal epithelial and carcinoma cells of human bladder were investigated for the cytokeratin which is one of the intermediate filaments and comprises cytoskeleton using the immunofluorescence method. Carcinoma cell lines used were JTC-30, JTC-32, HUB-41 and T-24. In normal urothelium, keratin fibers were fine and straight with unchanged diameter and distributed regularly in the cytoplasm. By contrast, keratin fibers in bladder carcinoma cells were kinked and changed in diameter and were distributed irregularly in the cytoplasm. The above findings were most obvious in T-24 which formed undifferentiated carcinoma when transplanted to nude mice, and keratin fibers were dominantly located in the perinuclear area. The changes of keratin fibers appeared to be parallel to the grade of histological anaplasia of the tumor formed by implantation of bladder carcinoma cell line cells to nude mice. These observations suggest that the morphology of cytokeratin is a useful indicator for evaluating the grade of malignancy in transitional cell carcinoma |
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| Description: | Date Completed 12.12.1990 Date Revised 15.11.2006 published: Print Citation Status MEDLINE |
| ISSN: | 0018-1994 |