A general method to cleave a known DNA sequence at any site
We describe a new method for obtaining DNA fragments starting at a desired point where there is no recognition sequence for any known restriction endonuclease. A single-stranded DNA containing the fragment of interest is annealed to a synthetic oligonucleotide hybridizing at the 5' end of the r...
| Veröffentlicht in: | BioTechniques. - 1991. - 11(1991), 4 vom: 30. Okt., Seite 432-4, 436 |
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| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1991
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Deoxyribonucleotides Epidermal Growth Factor 62229-50-9 DNA 9007-49-2 DNA Polymerase I EC 2.7.7.7 DNA Restriction Enzymes |