Methods to identify and avoid artifactual formation of interchain disulfide bonds when analyzing proteins by SDS-PAGE
Amicyanin is a monomeric protein of known structure which possesses a single cysteine that serves as a ligand to copper in its native state. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of amicyanin after denaturation in the presence and absence of beta-mercaptoethan...
| Veröffentlicht in: | BioTechniques. - 1991. - 12(1992), 2 vom: 01. Feb., Seite 198, 200, 202 |
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| Format: | Aufsatz |
| Sprache: | English |
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1992
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, U.S. Gov't, P.H.S. Bacterial Proteins Disulfides Proteins mauC protein, Methylobacterium extorquens Cystine 48TCX9A1VT Cysteine K848JZ4886 |