Application of the chloramphenicol acetyltransferase (CAT) diffusion assay to transgenic plant tissues
Chloramphenicol acetyltransferase (CAT) activity was quantified in crude extracts from tobacco callus tissues using a modification of a previously reported diffusion assay. We describe here the alterations necessary in applying this rapid and simple assay procedure to plant materials. Due to the hig...
Veröffentlicht in: | BioTechniques. - 1991. - 12(1992), 2 vom: 01. Feb., Seite 181-4, 186 |
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Format: | Aufsatz |
Sprache: | English |
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1992
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Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Plant Extracts Plant Proteins Recombinant Fusion Proteins Sulfhydryl Reagents Monophenol Monooxygenase EC 1.14.18.1 Chloramphenicol O-Acetyltransferase mehr... |