Efficient production of single-stranded DNA as long as 2 kb for sequencing of PCR-amplified DNA

Efficient production of single-stranded DNA as long as 2 kb for sequencing of PCR-amplified DNA

A modification of the asymmetric PCR method is described, which reliably facilitates sequencing of PCR-amplified DNA. This procedure produces single-stranded DNA fragments as long as two kilobases that are suitable for dideoxy DNA sequencing. First, a PCR for double-stranded DNA is preformed under o...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 12(1992), 2 vom: 01. Feb., Seite 164, 166, 168-71
1. Verfasser: Kaltenboeck, B (VerfasserIn)
Weitere Verfasser: Spatafora, J W, Zhang, X, Kousoulas, K G, Blackwell, M, Storz, J
Format: Aufsatz
Sprache:English
Veröffentlicht: 1992
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. DNA, Single-Stranded DNA 9007-49-2