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01000naa a22002652 4500 |
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NLM014966301 |
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DE-627 |
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20231221062154.0 |
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tu |
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231221s1992 xx ||||| 00| ||eng c |
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|a pubmed24n0050.xml
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|a (DE-627)NLM014966301
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|a (NLM)1515143
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Bronstein, I
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Improved chemiluminescent western blotting procedure
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| 264 |
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1 |
|c 1992
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 06.10.1992
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| 500 |
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|a Date Revised 21.11.2013
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| 500 |
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|a published: Print
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|a Citation Status MEDLINE
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|a A chemiluminescent Western blotting procedure and its application in assays for human transferrin and human immunodeficiency virus-I antibodies are described. The procedure is based on a chemiluminescent substrate, adamantyl 1,2-dioxetane aryl phosphate and alkaline phosphatase-labeled detection antibodies. Different membranes (polyvinylidene fluoride, nitrocellulose, nylon) and a proprietary membrane treatment agent (Nitro-Block) have been studied. This sensitive blotting procedure utilizing AMPPD, a polyclonal rabbit anti-transferrin:goat anti-rabbit IgG-alkaline phosphatase detection complex, and a PVDF membrane blocked with Nitro-Block permits the detection of 125 pg (1.6 fmol) of human transferrin. A novel 1,2-dioxetane substrate, CSPD, has also been evaluated
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a HIV Antibodies
|2 NLM
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| 650 |
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7 |
|a Membranes, Artificial
|2 NLM
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| 650 |
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7 |
|a Nylons
|2 NLM
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| 650 |
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7 |
|a Polyvinyls
|2 NLM
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| 650 |
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7 |
|a Transferrin
|2 NLM
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| 650 |
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7 |
|a 3-(2'-spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane
|2 NLM
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| 650 |
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7 |
|a 122341-56-4
|2 NLM
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| 650 |
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7 |
|a polyvinylidene fluoride
|2 NLM
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| 650 |
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7 |
|a 24937-79-9
|2 NLM
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| 650 |
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7 |
|a Collodion
|2 NLM
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| 650 |
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7 |
|a 9004-70-0
|2 NLM
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| 650 |
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7 |
|a Adamantane
|2 NLM
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| 650 |
|
7 |
|a PJY633525U
|2 NLM
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| 700 |
1 |
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|a Voyta, J C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Murphy, O J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bresnick, L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kricka, L J
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 12(1992), 5 vom: 01. Mai, Seite 748-53
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:12
|g year:1992
|g number:5
|g day:01
|g month:05
|g pages:748-53
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|d 12
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|h 748-53
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