Nonradioactive southwestern analysis using chemiluminescent detection
Replacing radioactively labeled probes by nonradioactive ones and detection by chemiluminescence instead of colorimetry allows a nonhazardous handling and offers the possibility of easily reprobing filters in Southwestern analysis. Using the described procedure, we were able to determine the molecul...
| Publié dans: | BioTechniques. - 1988. - 13(1992), 4 vom: 12. Okt., Seite 540-3 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
1992
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| Accès à la collection: | BioTechniques |
| Sujets: | Comparative Study Journal Article DNA-Binding Proteins Deoxyuracil Nucleotides Membranes, Artificial Oligonucleotide Probes Polyvinyls digoxigenin-11-deoxyuridine triphosphate polyvinylidene fluoride 24937-79-9 plus... |
| Résumé: | Replacing radioactively labeled probes by nonradioactive ones and detection by chemiluminescence instead of colorimetry allows a nonhazardous handling and offers the possibility of easily reprobing filters in Southwestern analysis. Using the described procedure, we were able to determine the molecular weight of DNA-binding proteins and achieve a high signal-to-noise ratio |
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| Description: | Date Completed 08.02.1993 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |