Quantitation of targets for PCR by use of limiting dilution

Quantitation of targets for PCR by use of limiting dilution

We describe a general method to quantitate the total number of initial targets present in a sample using limiting dilution, PCR and Poisson statistics. The DNA target for the PCR was the rearranged immunoglobulin heavy chain (IgH) gene derived from a leukemic clone that was quantitated against a bac...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 13(1992), 3 vom: 01. Sept., Seite 444-9
1. Verfasser: Sykes, P J (VerfasserIn)
Weitere Verfasser: Neoh, S H, Brisco, M J, Hughes, E, Condon, J, Morley, A A
Format: Aufsatz
Sprache:English
Veröffentlicht: 1992
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA, Neoplasm Immunoglobulin Heavy Chains