A simple procedure for recovering the denaturing effect of methylmercury in agarose gel electrophoresis

A simple and rapid procedure for recovering the denaturing effect of methylmercuric hydroxide in agarose gel electrophoresis is described. The procedure consisted of the treatment of the commercial methylmercuric hydroxide solutions with Amberlite, a mixture of anion- and cation-exchange resins. Thi...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 12(1992), 4 vom: 01. Apr., Seite 480-2
1. Verfasser: Fernández-Silva, P (VerfasserIn)
Weitere Verfasser: Enriquez, J A, Montoya, J
Format: Aufsatz
Sprache:English
Veröffentlicht: 1992
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Methylmercury Compounds Resins, Synthetic RNA 63231-63-0 amberlite 9079-25-8 methylmercury hydroxide EZ74BDI0HB
Beschreibung
Zusammenfassung:A simple and rapid procedure for recovering the denaturing effect of methylmercuric hydroxide in agarose gel electrophoresis is described. The procedure consisted of the treatment of the commercial methylmercuric hydroxide solutions with Amberlite, a mixture of anion- and cation-exchange resins. This treatment greatly improved the resolution of RNA species when fractionated by electrophoresis through agarose-CH3HgOH slab gel
Beschreibung:Date Completed 18.09.1992
Date Revised 19.11.2015
published: Print
Citation Status MEDLINE
ISSN:1940-9818