Biological and molecular characterization of the post-invasion immature stages of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)

We performed molecular and biological characterizations of immature stages of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) using insects from different states in Brazil, and different host plants. Three laboratory colonies of H. armigera were established with insects from the states of São P...

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Veröffentlicht in:The Florida Entomologist. in. - Florida Entomological Society. - 101(2018), 1, Seite 25-32
1. Verfasser: da Silva, Ivana Fernandes (VerfasserIn)
Weitere Verfasser: Baldin, Edson Luiz Lopes, Specht, Alexandre, Sosa-Gómez, Daniel Ricardo, Roque-Specht, Vânia Ferreira, Morando, Rafaela, de Paula-Moraes, Silvana Vieira
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:The Florida Entomologist. in
Schlagworte:Biological sciences Behavioral sciences
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520 |a We performed molecular and biological characterizations of immature stages of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) using insects from different states in Brazil, and different host plants. Three laboratory colonies of H. armigera were established with insects from the states of São Paulo, Bahia, and Distrito Federal, that were collected from citrus, cotton, and corn, respectively. For each colony, microsatellites were used to assess genetic similarity among insects. The biology of the immature stages from each colony also was compared under laboratory conditions (25 ± 1 °C, 70 ± 10% RH, 14:10 h [L:D] photoperiod). Microsatellite analysis revealed genetic variability in sampled specimens and that the grouping of individuals was independent of the geographic origin. The utility of an artificial diet was validated during the comparative biology experiment, and the ingredients of the artificial diet were analyzed for purity, particle size, and the presence and quantity of aggregates. The mean development times of the larvae and pre-pupae from the 3 locations were similar, and mean total development time (egg to adult) was not significantly different. Neste estudo foram realizadas a caracterização molecular e a comparação da biologia de estádios imaturos de Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Foram estabelecidas três colônias desta espécie, a partir da coleta de insetos oriundos dos estados de São Paulo, Bahia, e Distrito Federal, em plantas de citros, algodão e milho, respectivamente. Análises genética foram conduzidas usando microssatélites de forma a verificar a similaridade genética dos insetos de cada colônia. A biologia de imaturos foi estudada sob condições de laboratório (T = 25 ± 1 °C, UR = 70 ± 10% fotoperíodo = 14:10 h L:D). A análise de microssatélites revelou variabilidade genética nos espécimes amostrados e que o agrupamento dos indivíduos foi independente da origem geográfica. Uma dieta artificial foi utilizada e validada durante o estudo de biologia comparada. Amostras de cade um dos ingredientes da dieta artificial foram analisadas quanto à pureza, tamanho de partícula e presença e quantidade de agregados. As viabilidades médias das larvas e pré-pupas nas três populações foram semelhantes. E o tempo total de desenvolvimento total (ovo a adulto) não foi significativamente diferente. 
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650 4 |a Biological sciences  |x Biology  |x Developmental biology  |x Animal development  |x Insect development  |x Insect larvae 
650 4 |a Biological sciences  |x Agriculture  |x Agricultural sciences  |x Animal science  |x Animal nutrition  |x Animal feeding methods  |x Artificial diets 
650 4 |a Biological sciences  |x Agriculture  |x Agricultural sciences  |x Animal science  |x Animal husbandry  |x Animal genetics  |x Insect genetics 
650 4 |a Biological sciences  |x Biology  |x Zoology  |x Entomology 
650 4 |a Biological sciences  |x Ecology  |x Animal ecology  |x Insect ecology  |x Insect communities  |x Insect colonies 
650 4 |a Biological sciences  |x Biology  |x Developmental biology  |x Growth and development  |x Developmental stages  |x Immatures 
650 4 |a Biological sciences  |x Biology  |x Developmental biology  |x Growth and development  |x Developmental stages  |x Pupae 
650 4 |a Behavioral sciences  |x Ethology  |x Animal behavior  |x Animal feeding behavior  |x Feeding preferences 
650 4 |a Biological sciences  |x Biology  |x Developmental biology  |x Animal development  |x Larval development 
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700 1 |a Baldin, Edson Luiz Lopes  |e verfasserin  |4 aut 
700 1 |a Specht, Alexandre  |e verfasserin  |4 aut 
700 1 |a Sosa-Gómez, Daniel Ricardo  |e verfasserin  |4 aut 
700 1 |a Roque-Specht, Vânia Ferreira  |e verfasserin  |4 aut 
700 1 |a Morando, Rafaela  |e verfasserin  |4 aut 
700 1 |a de Paula-Moraes, Silvana Vieira  |e verfasserin  |4 aut 
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