Evolutionary repurposing of a sulfatase : A new Michaelis complex leads to efficient transition state charge offset
The recruitment and evolutionary optimization of promiscuous enzymes is key to the rapid adaptation of organisms to changing environments. Our understanding of the precise mechanisms underlying enzyme repurposing is, however, limited: What are the active-site features that enable the molecular recog...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | Proceedings of the National Academy of Sciences of the United States of America. - National Academy of Sciences of the United States of America. - 115(2018), 31, Seite E7293-E7302
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| 1. Verfasser: |
Miton, Charlotte M.
(VerfasserIn) |
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| Weitere Verfasser: |
Jonas, Stefanie,
Fischer, Gerhard,
Duarte, Fernanda,
Mohamed, Mark F.,
van Loo, Bert,
Kintses, Bálint,
Kamerlin, Shina C. L.,
Tokuriki, Nobuhiko,
Hyvönen, Marko,
Hollfelder, Florian |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2018
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| Zugriff auf das übergeordnete Werk: | Proceedings of the National Academy of Sciences of the United States of America
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| Schlagworte: | catalytic promiscuity
directed evolution
linear free-energy relationship
phosphate transfer
enzyme–substrate complementarity |
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