|
|
|
|
LEADER |
01000caa a22002652 4500 |
001 |
JST125307527 |
003 |
DE-627 |
005 |
20240625085614.0 |
007 |
cr uuu---uuuuu |
008 |
190103s2015 xx |||||o 00| ||en c |
035 |
|
|
|a (DE-627)JST125307527
|
035 |
|
|
|a (JST)newphytologist.206.1.303
|
040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
041 |
|
|
|a en
|
245 |
1 |
0 |
|a Sapogenin content variation in Medicago inter-specific hybrid derivatives highlights some aspects of saponin synthesis and control
|
264 |
|
1 |
|c 2015
|
336 |
|
|
|a Text
|b txt
|2 rdacontent
|
337 |
|
|
|a Computermedien
|b c
|2 rdamedia
|
338 |
|
|
|a Online-Ressource
|b cr
|2 rdacarrier
|
520 |
|
|
|a Summary In the Medicago genus, saponins are a complex mixture of triterpene glycosides showing a broad spectrum of biological properties. Here we analyzed the variation in the sapogenin content and composition of inter-specific hybrid Medicago sativa × Medicago arborea derivatives to highlight the pattern of this variation in plant organs (leaves/roots) and the possible mechanisms underlying it. In Sativa Arborea Cross (SAC) leaves and roots, saponins and sapogenins were evaluated using chromatographic methods. Phenotypic correlations between sapogenin content and bio-agronomic traits were examined. Expression studies on b-amyrin synthase and four cytochromes P450 (CYPs) involved in sapogenin biosynthesis and sequence analysis of the key gene of the hemolytic sapogenin pathway (CYP716A12) were performed. Chromatographic analyses revealed a different pattern of among-family variation for hemolytic and nonhemolytic sapogenins and saponins and for the two organs/tissues. Different correlation patterns of gene expression in roots and leaves were found. Diachronic analysis revealed a relationship between sapogenin content and gene transcriptional levels in the early stages of the productive cycle. The results suggest that there are different control mechanisms acting on sapogenin biosynthesis for leaves and roots, which are discussed. A key role for medicagenic acid in the control of sapogenin content in both the tissues is proposed and discussed.
|
540 |
|
|
|a © 2015 New Phytologist Trust
|
650 |
|
4 |
|a cytochrome P450 expression
|
650 |
|
4 |
|a inter-specific cross
|
650 |
|
4 |
|a Medicago arborea
|
650 |
|
4 |
|a Medicago sativa (alfalfa)
|
650 |
|
4 |
|a sapogenin synthesis
|
650 |
|
4 |
|a triterpene saponin
|
650 |
|
4 |
|a Biological sciences
|x Biochemistry
|x Biomolecules
|x Macromolecules
|x Carbohydrates
|x Glycoconjugates
|x Glycosides
|x Saponins
|x Sapogenins
|
650 |
|
4 |
|a Biological sciences
|x Biology
|x Botany
|x Plants
|
650 |
|
4 |
|a Biological sciences
|x Biochemistry
|x Biomolecules
|x Macromolecules
|x Carbohydrates
|x Glycoconjugates
|x Glycosides
|x Saponins
|
650 |
|
4 |
|a Biological sciences
|x Biology
|x Botany
|x Plant biology
|x Plant anatomy
|x Plant morphology
|x Plant organs
|x Leaves
|
650 |
|
4 |
|a Biological sciences
|x Biology
|x Botany
|x Plant biology
|x Plant anatomy
|x Plant morphology
|x Plant organs
|x Plant roots
|
650 |
|
4 |
|a Biological sciences
|x Agriculture
|x Agricultural sciences
|x Agronomy
|x Crops
|x Field crops
|x Food crops
|x Grass crops
|x Alfalfa
|
650 |
|
4 |
|a Biological sciences
|x Biochemistry
|x Metabolism
|x Biosynthesis
|
650 |
|
4 |
|a Biological sciences
|x Biology
|x Botany
|x Plant biology
|x Plant anatomy
|x Plant morphology
|x Plant organs
|x Stems
|
650 |
|
4 |
|a Social sciences
|x Sociology
|x Human societies
|x Social institutions
|x Families
|x Family members
|x Parents
|
650 |
|
4 |
|a Health sciences
|x Medical conditions
|x Diseases
|x Neoplasia
|x Nodules
|x Research
|x Full papers
|
655 |
|
4 |
|a research-article
|
773 |
0 |
8 |
|i Enthalten in
|t New Phytologist
|d New Phytologist Trust
|g 206(2015), 1, Seite 303-314
|w (DE-627)268132674
|w (DE-600)1472194-6
|x 14698137
|7 nnns
|
773 |
1 |
8 |
|g volume:206
|g year:2015
|g number:1
|g pages:303-314
|
856 |
4 |
0 |
|u https://www.jstor.org/stable/newphytologist.206.1.303
|3 Volltext
|
912 |
|
|
|a GBV_USEFLAG_A
|
912 |
|
|
|a SYSFLAG_A
|
912 |
|
|
|a GBV_JST
|
912 |
|
|
|a GBV_ILN_11
|
912 |
|
|
|a GBV_ILN_20
|
912 |
|
|
|a GBV_ILN_22
|
912 |
|
|
|a GBV_ILN_23
|
912 |
|
|
|a GBV_ILN_24
|
912 |
|
|
|a GBV_ILN_31
|
912 |
|
|
|a GBV_ILN_32
|
912 |
|
|
|a GBV_ILN_39
|
912 |
|
|
|a GBV_ILN_40
|
912 |
|
|
|a GBV_ILN_60
|
912 |
|
|
|a GBV_ILN_62
|
912 |
|
|
|a GBV_ILN_63
|
912 |
|
|
|a GBV_ILN_65
|
912 |
|
|
|a GBV_ILN_69
|
912 |
|
|
|a GBV_ILN_70
|
912 |
|
|
|a GBV_ILN_73
|
912 |
|
|
|a GBV_ILN_74
|
912 |
|
|
|a GBV_ILN_90
|
912 |
|
|
|a GBV_ILN_95
|
912 |
|
|
|a GBV_ILN_100
|
912 |
|
|
|a GBV_ILN_105
|
912 |
|
|
|a GBV_ILN_110
|
912 |
|
|
|a GBV_ILN_120
|
912 |
|
|
|a GBV_ILN_138
|
912 |
|
|
|a GBV_ILN_150
|
912 |
|
|
|a GBV_ILN_151
|
912 |
|
|
|a GBV_ILN_161
|
912 |
|
|
|a GBV_ILN_170
|
912 |
|
|
|a GBV_ILN_171
|
912 |
|
|
|a GBV_ILN_187
|
912 |
|
|
|a GBV_ILN_211
|
912 |
|
|
|a GBV_ILN_213
|
912 |
|
|
|a GBV_ILN_224
|
912 |
|
|
|a GBV_ILN_230
|
912 |
|
|
|a GBV_ILN_266
|
912 |
|
|
|a GBV_ILN_285
|
912 |
|
|
|a GBV_ILN_293
|
912 |
|
|
|a GBV_ILN_370
|
912 |
|
|
|a GBV_ILN_374
|
912 |
|
|
|a GBV_ILN_381
|
912 |
|
|
|a GBV_ILN_602
|
912 |
|
|
|a GBV_ILN_636
|
912 |
|
|
|a GBV_ILN_647
|
912 |
|
|
|a GBV_ILN_702
|
912 |
|
|
|a GBV_ILN_2001
|
912 |
|
|
|a GBV_ILN_2003
|
912 |
|
|
|a GBV_ILN_2004
|
912 |
|
|
|a GBV_ILN_2005
|
912 |
|
|
|a GBV_ILN_2006
|
912 |
|
|
|a GBV_ILN_2007
|
912 |
|
|
|a GBV_ILN_2008
|
912 |
|
|
|a GBV_ILN_2009
|
912 |
|
|
|a GBV_ILN_2010
|
912 |
|
|
|a GBV_ILN_2011
|
912 |
|
|
|a GBV_ILN_2014
|
912 |
|
|
|a GBV_ILN_2015
|
912 |
|
|
|a GBV_ILN_2018
|
912 |
|
|
|a GBV_ILN_2020
|
912 |
|
|
|a GBV_ILN_2021
|
912 |
|
|
|a GBV_ILN_2025
|
912 |
|
|
|a GBV_ILN_2026
|
912 |
|
|
|a GBV_ILN_2027
|
912 |
|
|
|a GBV_ILN_2031
|
912 |
|
|
|a GBV_ILN_2034
|
912 |
|
|
|a GBV_ILN_2037
|
912 |
|
|
|a GBV_ILN_2038
|
912 |
|
|
|a GBV_ILN_2039
|
912 |
|
|
|a GBV_ILN_2044
|
912 |
|
|
|a GBV_ILN_2048
|
912 |
|
|
|a GBV_ILN_2049
|
912 |
|
|
|a GBV_ILN_2050
|
912 |
|
|
|a GBV_ILN_2055
|
912 |
|
|
|a GBV_ILN_2056
|
912 |
|
|
|a GBV_ILN_2057
|
912 |
|
|
|a GBV_ILN_2059
|
912 |
|
|
|a GBV_ILN_2061
|
912 |
|
|
|a GBV_ILN_2064
|
912 |
|
|
|a GBV_ILN_2068
|
912 |
|
|
|a GBV_ILN_2088
|
912 |
|
|
|a GBV_ILN_2093
|
912 |
|
|
|a GBV_ILN_2106
|
912 |
|
|
|a GBV_ILN_2107
|
912 |
|
|
|a GBV_ILN_2108
|
912 |
|
|
|a GBV_ILN_2110
|
912 |
|
|
|a GBV_ILN_2111
|
912 |
|
|
|a GBV_ILN_2112
|
912 |
|
|
|a GBV_ILN_2113
|
912 |
|
|
|a GBV_ILN_2118
|
912 |
|
|
|a GBV_ILN_2119
|
912 |
|
|
|a GBV_ILN_2122
|
912 |
|
|
|a GBV_ILN_2129
|
912 |
|
|
|a GBV_ILN_2143
|
912 |
|
|
|a GBV_ILN_2144
|
912 |
|
|
|a GBV_ILN_2147
|
912 |
|
|
|a GBV_ILN_2148
|
912 |
|
|
|a GBV_ILN_2152
|
912 |
|
|
|a GBV_ILN_2153
|
912 |
|
|
|a GBV_ILN_2188
|
912 |
|
|
|a GBV_ILN_2190
|
912 |
|
|
|a GBV_ILN_2232
|
912 |
|
|
|a GBV_ILN_2336
|
912 |
|
|
|a GBV_ILN_2360
|
912 |
|
|
|a GBV_ILN_2470
|
912 |
|
|
|a GBV_ILN_2472
|
912 |
|
|
|a GBV_ILN_2507
|
912 |
|
|
|a GBV_ILN_2522
|
912 |
|
|
|a GBV_ILN_2548
|
912 |
|
|
|a GBV_ILN_2939
|
912 |
|
|
|a GBV_ILN_2942
|
912 |
|
|
|a GBV_ILN_2946
|
912 |
|
|
|a GBV_ILN_2949
|
912 |
|
|
|a GBV_ILN_2951
|
912 |
|
|
|a GBV_ILN_4012
|
912 |
|
|
|a GBV_ILN_4035
|
912 |
|
|
|a GBV_ILN_4037
|
912 |
|
|
|a GBV_ILN_4046
|
912 |
|
|
|a GBV_ILN_4112
|
912 |
|
|
|a GBV_ILN_4125
|
912 |
|
|
|a GBV_ILN_4126
|
912 |
|
|
|a GBV_ILN_4242
|
912 |
|
|
|a GBV_ILN_4246
|
912 |
|
|
|a GBV_ILN_4249
|
912 |
|
|
|a GBV_ILN_4251
|
912 |
|
|
|a GBV_ILN_4305
|
912 |
|
|
|a GBV_ILN_4306
|
912 |
|
|
|a GBV_ILN_4307
|
912 |
|
|
|a GBV_ILN_4313
|
912 |
|
|
|a GBV_ILN_4322
|
912 |
|
|
|a GBV_ILN_4323
|
912 |
|
|
|a GBV_ILN_4324
|
912 |
|
|
|a GBV_ILN_4325
|
912 |
|
|
|a GBV_ILN_4326
|
912 |
|
|
|a GBV_ILN_4333
|
912 |
|
|
|a GBV_ILN_4334
|
912 |
|
|
|a GBV_ILN_4335
|
912 |
|
|
|a GBV_ILN_4336
|
912 |
|
|
|a GBV_ILN_4338
|
912 |
|
|
|a GBV_ILN_4346
|
912 |
|
|
|a GBV_ILN_4367
|
912 |
|
|
|a GBV_ILN_4393
|
912 |
|
|
|a GBV_ILN_4700
|
951 |
|
|
|a AR
|
952 |
|
|
|d 206
|j 2015
|e 1
|h 303-314
|