Viable Deletion Mutants in the Simian Virus 40 Early Region

Viable Deletion Mutants in the Simian Virus 40 Early Region

For the purpose of isolating hr-t-like mutants of simian virus 40, we have constructed variants that have lost the unique site for the restriction enzyme Taq I at 0.565. Five mutants have been isolated and characterized by restriction enzyme analysis. All of them produce a normal size T antigen. Fou...

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Veröffentlicht in:Proceedings of the National Academy of Sciences of the United States of America. - National Academy of Sciences of the United States of America. - 75(1978), 9, Seite 4455-4459
1. Verfasser: Feunteun, Jean (VerfasserIn)
Weitere Verfasser: Kress, Michel, Gardes, Maryvonne, Monier, Roger
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 1978
Zugriff auf das übergeordnete Werk:Proceedings of the National Academy of Sciences of the United States of America
Schlagworte:Genetics Taq I site T antigens Transformation Health sciences Applied sciences Biological sciences Physical sciences
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520 |a For the purpose of isolating hr-t-like mutants of simian virus 40, we have constructed variants that have lost the unique site for the restriction enzyme Taq I at 0.565. Five mutants have been isolated and characterized by restriction enzyme analysis. All of them produce a normal size T antigen. Four produce a t antigen reduced in size as well as in amount; the fifth one does not seem to make any t antigen at all. The ability of these mutants to transform mouse cells in vitro, as tested by anchorage dependence, is clearly altered; however, the defect is only partial. In the same test, the mutants can complement a tsA mutant for transformation and therefore define a second complementation group in the simian virus 40 early region. 
650 4 |a Genetics 
650 4 |a Taq I site 
650 4 |a T antigens 
650 4 |a Transformation 
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650 4 |a Physical sciences  |x Chemistry  |x Chemical compounds  |x Chemicals  |x Polymers  |x Biopolymers  |x Proteins  |x Enzymes 
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700 1 |a Gardes, Maryvonne  |e verfasserin  |4 aut 
700 1 |a Monier, Roger  |e verfasserin  |4 aut 
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