DNA Transfer of Focus- and Tumor-Forming Ability into Nontumorigenic CHEF Cells

DNA Transfer of Focus- and Tumor-Forming Ability into Nontumorigenic CHEF Cells

CHEF/18 fibroblastic cells derived from a Chinese hamster embryo are diploid and nontumorigenic and require multiple steps of chemical treatment and selection to produce tumorigenic derivatives. In this report, CHEF/18 cells and a mutant capable of growing in medium with a low concentration of serum...

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Veröffentlicht in:Proceedings of the National Academy of Sciences of the United States of America. - National Academy of Sciences of the United States of America. - 79(1982), 6, Seite 1964-1968
1. Verfasser: Smith, Barbara L. (VerfasserIn)
Weitere Verfasser: Anisowicz, Anthony, Chodosh, Lewis A., Sager, Ruth
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 1982
Zugriff auf das übergeordnete Werk:Proceedings of the National Academy of Sciences of the United States of America
Schlagworte:Genetics Transformation Human Cancer Transfection Chinese Hamster Cells Physical sciences Biological sciences Health sciences Business
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520 |a CHEF/18 fibroblastic cells derived from a Chinese hamster embryo are diploid and nontumorigenic and require multiple steps of chemical treatment and selection to produce tumorigenic derivatives. In this report, CHEF/18 cells and a mutant capable of growing in medium with a low concentration of serum, LS1-1, were recipients in DNA transfer experiments using the calcium phosphate coprecipitation method. Focus formation with donor DNAs from tumor-derived CHEF cells and from human bladder carcinoma cell line EJ gave yields of 0.02-0.59 focus per μ g of DNA per 106recipients. In one experiment in which CHEF/18 cells were transfected with EJ DNA, the presence of human DNA was detected in five of seven foci by using a cloned Alu sequence. Cells from one of these foci gave rise to tumors in nude mice, and the DNA produced secondary CHEF/ 18 transfectants. Because normal human cells as well as CHEF/ 18 cells require multiple stages to become tumorigenic, these findings suggest that EJ cells contain tumor-inducing DNA as the result of prior changes that occurred during the development of this carcinoma. 
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700 1 |a Sager, Ruth  |e verfasserin  |4 aut 
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