Restriction Point Control of Cell Growth by a Labile Protein: Evidence for Increased Stability in Transformed Cells

It has been proposed that animal cells must accumulate a labile protein(s) before they can pass the restriction (R) point in the G1phase of the cell cycle [Rossow, P. W., Riddle, V. G. H. & Pardee, A. B. (1979) Proc. Natl. Acad. Sci. USA 76, 4446-4450]. Here, we present evidence that this R prot...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:	Proceedings of the National Academy of Sciences of the United States of America. - National Academy of Sciences of the United States of America. - 79(1982), 2, Seite 436-440
1. Verfasser:	Campisi, Judith (VerfasserIn)
Weitere Verfasser:	Medrano, Estela E., Morreo, Gail, Pardee, Arthur B.
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	1982
Zugriff auf das übergeordnete Werk:	Proceedings of the National Academy of Sciences of the United States of America
Schlagworte:	Cell Biology Animal Cells Transformation Protein Degradation Cell Cycle Growth Control Biological sciences Physical sciences


LEADER	01000caa a22002652 4500
001	JST069526664
003	DE-627
005	20240622182637.0
007	cr uuu---uuuuu
008	150325s1982 xx \|\|\|\|\|o 00\| \|\|eng c
035			\|a (DE-627)JST069526664
035			\|a (JST)12091
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
100	1		\|a Campisi, Judith \|e verfasserin \|4 aut
245	1	0	\|a Restriction Point Control of Cell Growth by a Labile Protein: Evidence for Increased Stability in Transformed Cells
264		1	\|c 1982
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a It has been proposed that animal cells must accumulate a labile protein(s) before they can pass the restriction (R) point in the G1phase of the cell cycle [Rossow, P. W., Riddle, V. G. H. & Pardee, A. B. (1979) Proc. Natl. Acad. Sci. USA 76, 4446-4450]. Here, we present evidence that this R protein acquires increased stability in transformed 3T3 cells, thereby allowing these cells to continue growth under conditions that arrest untransformed cells. Low doses of cycloheximide or histidinol drastically reduced the rate at which normal 3T3 (A31) fibroblasts in early G1could enter DNA synthesis. These drugs had less effect on entry of two tumorigenic A31 derivatives, BPA31 and SVA31, into S, although measurement of [3H]leucine incorporation showed that the inhibitors were equally effective in the three cell lines. The hypothesis is that the transformed lines are less sensitive because moderate inhibition of their R protein synthesis is compensated by lower rates of protein degradation. To test this idea, we completely inhibited cytoplasmic protein synthesis for several hours shortly before A31 and BPA31 cells had reached the R point. After removal of inhibitor, A31 cells showed delays in the onset of S that were in excess of the inhibitor pulse, consistent with decay of labile protein during the pulse. BPA31 cells showed no excess delays, suggesting a much more stable R protein. The half-life of the R protein was estimated as 2.5 hr in A31 cells, indicating that, in these cells, R protein synthesis starts at the beginning of G1. In the BPA31 cells, the R protein showed no signs of decay for at least 8 hr.
650		4	\|a Cell Biology
650		4	\|a Animal Cells
650		4	\|a Transformation
650		4	\|a Protein Degradation
650		4	\|a Cell Cycle
650		4	\|a Growth Control
650		4	\|a Biological sciences \|x Biology \|x Cytology \|x Cell biology \|x Cells \|x Cultured cells \|x Cell lines \|x 3T3 cells
650		4	\|a Biological sciences \|x Biology \|x Cytology \|x Cell biology \|x Cells \|x Cultured cells \|x Cell lines
650		4	\|a Biological sciences \|x Biology \|x Genetics \|x Molecular genetics \|x Genetic translation \|x Protein synthesis
650		4	\|a Biological sciences \|x Biology \|x Cytology \|x Cell biology \|x Cells \|x Animal cells
650		4	\|a Biological sciences \|x Biology \|x Cytology \|x Cell biology \|x Cell physiology \|x Cell cycle
650		4	\|a Biological sciences \|x Biology \|x Cytology \|x Cell biology \|x Cell physiology \|x Cell growth
650		4	\|a Physical sciences \|x Chemistry \|x Chemical compounds \|x Chemicals \|x Acids \|x Nucleic acids \|x DNA
650		4	\|a Biological sciences \|x Biology \|x Cytology \|x Cell biology \|x Cells \|x Cultured cells \|x Cell lines \|x Transformed cell line
650		4	\|a Physical sciences \|x Physics \|x Microphysics \|x Atomic physics \|x Half lives
650		4	\|a Biological sciences \|x Biology \|x Cytology \|x Cell biology \|x Cells \|x Cultured cells
655		4	\|a research-article
700	1		\|a Medrano, Estela E. \|e verfasserin \|4 aut
700	1		\|a Morreo, Gail \|e verfasserin \|4 aut
700	1		\|a Pardee, Arthur B. \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Proceedings of the National Academy of Sciences of the United States of America \|d National Academy of Sciences of the United States of America \|g 79(1982), 2, Seite 436-440 \|w (DE-627)254235379 \|w (DE-600)1461794-8 \|x 10916490 \|7 nnns
773	1	8	\|g volume:79 \|g year:1982 \|g number:2 \|g pages:436-440
856	4	0	\|u https://www.jstor.org/stable/12091 \|3 Volltext
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_JST
912			\|a GBV_ILN_11
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_168
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_252
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_374
912			\|a GBV_ILN_381
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2018
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2360
912			\|a GBV_ILN_2943
912			\|a GBV_ILN_2946
912			\|a GBV_ILN_2949
912			\|a GBV_ILN_2951
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4346
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 79 \|j 1982 \|e 2 \|h 436-440