Somatic embryogenesis and plant regeneration in two wild cotton species belong to G genome

The present work describes the plant regeneration via somatic embryogenesis in two wild cotton species belonging to G genome: Gossypium nelsonii Fryx and Gossypium australe F Muell. The role of plant hormones and carbohydrates was also evaluated for somatic embryogenesis and somatic embryo developme...

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Veröffentlicht in:In Vitro Cellular & Developmental Biology. Plant. - Springer Science + Business Media, LLC, 1991. - 46(2010), 3, Seite 298-305
1. Verfasser: Yan, Shu-Feng (VerfasserIn)
Weitere Verfasser: Zhang, Qiao, Wang, Jing-Er, Sun, Yu-Qiang, Daud, M. K., Zhu, Shui-Jin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:In Vitro Cellular & Developmental Biology. Plant
Schlagworte:Biological sciences Physical sciences
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520 |a The present work describes the plant regeneration via somatic embryogenesis in two wild cotton species belonging to G genome: Gossypium nelsonii Fryx and Gossypium australe F Muell. The role of plant hormones and carbohydrates was also evaluated for somatic embryogenesis and somatic embryo development. Normal plants were obtained from G. nelsonii Fryx; abnormal plants and somatic embryos were obtained from G. australe F Muell. The best medium for callus induction for these G genome wild cotton species was MSB 5 supplemented with 0.1 mg L⁻¹ KT and 0.1 mg L⁻¹ 2,4-D. For embryogénie callus proliferation, the best medium used was MSB 5 supplemented with 0.2 mg L⁻¹ KT and 0.5 mg L⁻¹ IBA. The medium MSB 5 supplemented with 0.15 mg L⁻¹ KT and 0.5 mg L⁻¹ NAA was used successfully for root initiation and plant growth. In addition, adding CuSO₄ and AgNO₃ in the callus-inducing and proliferation medium resulted in a number of somatic embryos. Glucose and maltose, the carbon sources in somatic culture, were used for callus induction, but maltose worked even better than glucose for proliferation of embryogénie callus and development of somatic embryos. 
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650 4 |a Biological sciences  |x Agriculture  |x Agricultural sciences  |x Agronomy  |x Crops  |x Field crops  |x Fiber crops  |x Cotton 
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700 1 |a Sun, Yu-Qiang  |e verfasserin  |4 aut 
700 1 |a Daud, M. K.  |e verfasserin  |4 aut 
700 1 |a Zhu, Shui-Jin  |e verfasserin  |4 aut 
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