Spatiotemporal expression of lpa1 and lpa2 genes showed low phytate accumulation in maize foliage and seeds during early developmental stages
Copyright © 2025 Elsevier Masson SAS. All rights reserved.
| Publié dans: | Plant physiology and biochemistry : PPB. - 1991. - 229(2025), Pt C vom: 15. Okt., Seite 110627 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
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| Accès à la collection: | Plant physiology and biochemistry : PPB |
| Sujets: | Journal Article Developmental stages Eutrophication Fodder maize Maize Micronutrients Phytic acid Spatiotemporal expression |
| Résumé: | Copyright © 2025 Elsevier Masson SAS. All rights reserved. High phytate intake reduces the bioavailability of iron and zinc, coupled with its indigestibility in nonruminants, ultimately contributing to eutrophication. Here, we examined the spatiotemporal expression of two low-phytate genes, lpa1, and lpa2 in double (lpa1lpa2) and single (lpa1 and lpa2) mutants including wild types (lpa1+lpa2+), across seven diverse genetic backgrounds to decipher phytic acid phosphorus (PAP) accumulation patterns in foliage and seeds at three developmental stages [15, 30 and 45 days after pollination (DAP)], aiming to optimize harvest stage having lowest PAP for nutrient-rich maize. Wild types exhibited higher PAP levels (2.47-2.97 mg/g) compared to double (1.31-1.73 mg/g), lpa1 (1.43-1.86 mg/g), and lpa2 (1.57-2.12 mg/g) mutants in seeds from 15 to 45 DAP. In wild types, seed inorganic phosphorus (iP) levels decreased with maturity, indicating iP translocation for PAP synthesis, unlike mutants with stable iP concentrations. Conversely, in foliage, PAP and iP levels were statistically similar across developmental stages in each genotypic class. Real-time PCR confirmed lower expression of lpa1 (4.05-fold) and lpa2 (2.90-fold) genes in double mutants at 15 DAP, aligning with seed PAP trends. In contrast, gene expression in foliage remained unchanged across developmental stages in mutants and wild types, highlighting the seed-specific expression of lpa1 and lpa2 genes. These findings are crucial for modelling PAP levels to enhance maize nutritional quality for food, feed, and fodder, while addressing eutrophication concerns sustainably |
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| Description: | Date Revised 25.10.2025 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2025.110627 |