Method Comparison and Investigation of Interference Variables of a Hand-Held Hemoglobinometer(HemoCue Hb 201+) in Cats
© 2025 The Author(s). Veterinary Clinical Pathology published by Wiley Periodicals LLC on behalf of American Society for Veterinary Clinical Pathology.
| Veröffentlicht in: | Veterinary clinical pathology. - 1975. - 54(2025), 3 vom: 16. Sept., Seite 211-220 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
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| Zugriff auf das übergeordnete Werk: | Veterinary clinical pathology |
| Schlagworte: | Journal Article Comparative Study ADVIA 2120 anemia blood feline hemoglobin point‐of‐care testing Hemoglobins |
| Zusammenfassung: | © 2025 The Author(s). Veterinary Clinical Pathology published by Wiley Periodicals LLC on behalf of American Society for Veterinary Clinical Pathology. BACKGROUND: Regular blood sampling to monitor RBC mass in anemic cats can exacerbate anemia. Laboratory-based reporting can delay clinical decisions. A hand-held hemoglobinometer, HemoCue Hb 201+ (HC-201), requires only one drop of blood (10 μL) and provides results within 1 min OBJECTIVES: This preliminary study aimed to evaluate the utility of HC-201 in cats and investigate the impact of potential interferents on its performance METHODS: One hundred and fifty-four venous blood samples in EDTA from 93 cats were analyzed. Hemoglobin concentration was measured once using an ADVIA 2120 analyzer and compared to the mean of 2-3 replicate measurements from an HC-201. Agreement and systematic bias between HC-201 and ADVIA results, along with precision between HC-201 replicates, were assessed using Lin's concordance correlation coefficient, non-parametric Bland-Altman, Passing-Bablok regression, and intraclass correlation coefficient. The performance of HC-201 in the presence of anemia, leukocytosis, azotemia, lipemia, icterus, hemolysis, and peripheral versus jugular venipunctures was assessed using Wilcoxon rank-sum tests RESULTS: Passing-Bablok analysis revealed a significant constant bias (intercept = -2.242, 95% CI: -4.042 to -0.667) but no significant proportional bias (slope = 1.015, 95% CI: 1.000-1.032). HC-201 demonstrated excellent agreement (ρc = 0.989) and precision (ICC = 0.997) with a median bias of -0.67 g/L (p = 0.001). The total observed error was 3.02%, within the allowable limits defined by international standards. Neither anemia, leukocytosis, azotemia, lipemia, nor venipuncture site influenced HC-201 measurements. Samples with icterus and hemolysis were insufficient in number for statistical comparison CONCLUSIONS: This preliminary study indicates that HC-201 offers reliable point-of-care monitoring for hemoglobin concentration in cats |
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| Beschreibung: | Date Completed 17.09.2025 Date Revised 20.09.2025 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1939-165X |
| DOI: | 10.1111/vcp.70035 |