Continuous light induces adventitious root formation in Robinia pseudoacacia L. through the auxin response factor RpARF12 lacking the CTD domain

Continuous light induces adventitious root formation in Robinia pseudoacacia L. through the auxin response factor RpARF12 lacking the CTD domain

Copyright © 2025. Published by Elsevier Masson SAS.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 226(2025) vom: 19. Mai, Seite 110049
1. Verfasser: Wu, Yue (VerfasserIn)
Weitere Verfasser: Guo, Qi, Cheng, Yizhe, Zhang, Shumin, Yu, Lixin, El-Kassaby, Yousry A, Zhao, Ye, Zhang, Man, Han, Kunjin, He, Yuxin, Luo, Haoxuan, Hu, Shuqing, Li, Yun, Sun, Yuhan
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2025
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Auxin signaling Continuous light Robinia pseudoacacia Root formation RpARF12
Beschreibung
Zusammenfassung:Copyright © 2025. Published by Elsevier Masson SAS.
We report a novel mechanism in adventitious root formation, where a non-canonical auxin response factor, RpARF12, uniquely integrates continuous light (CL) signals with auxin pathways to regulate adventitious root formation in Robinia pseudoacacia L. (black locust). Unlike typical ARFs, the RpARF12 lacks the C-terminal dimerization (CTD) domain, suggesting its function through auxin-independent regulatory pathways. Our comprehensive analysis revealed that RpARF12 expression is significantly induced by CL and plays a crucial role in adventitious root formation under these conditions. Heterologous transformation of RpARF12 into model plants, including Arabidopsis thaliana, Nicotiana tabacum, and Populus alba × P. glandulosa (poplar 84K), significantly promoted adventitious root formation, particularly under CL. Additionally, the application of exogenous auxin further increased the number and length of roots in RpARF12-overexpressed A. thaliana plants under CL by 1.60 and 1.37 times, respectively, compared to wild-type plants. The auxin polar transport inhibitor NPA decreased root formation in the RpARF12-overexpressed A. thaliana plants under CL conditions. This study provides valuable insights into the regulatory role of photoperiod, specifically CL, on adventitious root formation and highlights the important role of RpARF12 in this process
Beschreibung:Date Revised 25.05.2025
published: Print-Electronic
Citation Status Publisher
ISSN:1873-2690
DOI:10.1016/j.plaphy.2025.110049