Functional characterization of MinisST1.2, a sulfate transporter gene family member, on absorption and accumulation of Cr(VI) in Miscanthus sinensis

Copyright © 2025 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 225(2025) vom: 19. Mai, Seite 110033
1. Verfasser: Nie, Gang (VerfasserIn)
Weitere Verfasser: He, Jie, Liu, Aiyu, Zhou, Jie, Bi, Ming, Lai, Huiqi, Ma, Jieyu, Wang, Xia, Feng, Guangyan, Huang, Linkai, Zhang, Xinquan
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2025
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Functional analysis Heavy metal chromium Miscanthus sinensis ST gene family Sulfate transporter
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520 |a Soil contamination with heavy metals, particularly hexavalent chromium, represents a critical global environmental challenge. Miscanthus sinensis, a perennial C4 grass species, exhibits exceptional heavy metal tolerance while maintaining robust biomass production under Cr stress. This study presents the first genome-wide characterization of the sulfate transporter (ST) gene family in M. sinensis, with focused investigation of MisinST1.2 functionality. Transcriptional profiling revealed spatiotemporal specificity in MisinST1.2 expression across treatment durations and different tissue types. Heterologous overexpression in yeast systems unexpectedly enhanced Cr(VI) sensitivity, as evidenced by impaired growth under Cr(VI) exposure compared to wild-type controls. Parallel experiments in Arabidopsis thaliana demonstrated exacerbated stress responses in MisinST1.2-overexpressing lines relative to wild-type. Notably, transgenic line OE3 accumulated 7.75 mg/kg and 23.50 mg/kg Cr concentrations in above and underground tissues respectively, higher than 1.43 mg/kg and 10.63 mg/kg in WT. These findings elucidate the pivotal role of MisinST1.2 in mediating Cr uptake and accumulation strategies in M. sinensis. Our results advance understanding of plant heavy metal transport mechanisms while establishing a molecular framework for optimizing phytoremediation strategies in heavy metal-contaminated soils 
650 4 |a Journal Article 
650 4 |a Functional analysis 
650 4 |a Heavy metal chromium 
650 4 |a Miscanthus sinensis 
650 4 |a ST gene family 
650 4 |a Sulfate transporter 
700 1 |a He, Jie  |e verfasserin  |4 aut 
700 1 |a Liu, Aiyu  |e verfasserin  |4 aut 
700 1 |a Zhou, Jie  |e verfasserin  |4 aut 
700 1 |a Bi, Ming  |e verfasserin  |4 aut 
700 1 |a Lai, Huiqi  |e verfasserin  |4 aut 
700 1 |a Ma, Jieyu  |e verfasserin  |4 aut 
700 1 |a Wang, Xia  |e verfasserin  |4 aut 
700 1 |a Feng, Guangyan  |e verfasserin  |4 aut 
700 1 |a Huang, Linkai  |e verfasserin  |4 aut 
700 1 |a Zhang, Xinquan  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 225(2025) vom: 19. Mai, Seite 110033  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnas 
773 1 8 |g volume:225  |g year:2025  |g day:19  |g month:05  |g pages:110033 
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