Porphyrin-Based Metal-Organic Frameworks for PD-L1 Detection via "Coordination Disaggregation-Induced Enhancement" Strategy
Porphyrins, known for their exceptional photoelectrochemical properties and high luminescence, are promising candidates for electrochemiluminescence (ECL) applications. However, their tendency to aggregate in aqueous solutions due to π-π stacking leads to luminescence quenching and reduced efficienc...
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 41(2025), 10 vom: 18. März, Seite 6894-6902 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
|
| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
| Sujets: | Journal Article Metal-Organic Frameworks Porphyrins B7-H1 Antigen CD274 protein, human |
| Résumé: | Porphyrins, known for their exceptional photoelectrochemical properties and high luminescence, are promising candidates for electrochemiluminescence (ECL) applications. However, their tendency to aggregate in aqueous solutions due to π-π stacking leads to luminescence quenching and reduced efficiency. To address this, we developed a "coordination disaggregation-induced enhancement" strategy, utilizing metal-organic frameworks (MOFs) as stable platforms for immobilizing porphyrin. These porphyrin-based MOFs not only increase the loading of luminescent groups and suppress the aggregation-caused quenching (ACQ) effect but also enhance electron transfer via uniform dispersion of pyrrole N in the porphyrin ligand, thereby improving ECL intensity. Additionally, they exhibit favorable biocompatibility and low toxicity, making them suitable for biomedical applications. By combining porphyrin-based MOFs as donors with PDAMnO2 composites as quenchers, we constructed a quenching-type ECL immunosensor for detecting programmed death ligand 1 (PD-L1). This sensor achieves a detection range of 10 fg/mL to 100 ng/mL, with a limit of detection as low as 2.48 fg/mL, which demonstrates great potential for highly sensitive biomarker detection and promising applications in early cancer diagnosis and other medical diagnostics |
|---|---|
| Description: | Date Completed 12.05.2025 Date Revised 12.05.2025 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/acs.langmuir.4c05144 |