AtPHR1 and SlPHL1 positively regulate phosphorus remobilization in response to phosphate starvation by directly facilitating the transcription of genes encoding S-like ribonucleases in Arabidopsis and tomato, respectively

AtPHR1 and SlPHL1 positively regulate phosphorus remobilization in response to phosphate starvation by directly facilitating the transcription of genes encoding S-like ribonucleases in Arabidopsis and tomato, respectively

Copyright © 2025 Elsevier Masson SAS. All rights reserved.

Détails bibliographiques
Publié dans:Plant physiology and biochemistry : PPB. - 1991. - 221(2025) vom: 15. Apr., Seite 109608
Auteur principal: Li, Chengquan (Auteur)
Autres auteurs: Huang, Shaoxuan, Zhang, Duanmei, Zhu, Lin, Chen, Mingxue, Liu, Yanan, Zhang, Lang, Yang, Jie, Zhang, Xinyao, Yu, Yang, Li, Yanxia, An, Wenhao, Yang, Shuyue, Zhang, Yongqiang, Liu, Zhongjuan
Format: Article en ligne
Langue:English
Publié: 2025
Accès à la collection:Plant physiology and biochemistry : PPB
Sujets:Journal Article Arabidopsis AtPHR1 Phosphate starvation Phosphorus remobilization S-like RNase SlPHL1 Solanum lycopersicum Phosphorus 27YLU75U4W plus... Phosphates Arabidopsis Proteins Plant Proteins Ribonucleases EC 3.1.- Transcription Factors PHR1 protein, Arabidopsis
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245 1 0 |a AtPHR1 and SlPHL1 positively regulate phosphorus remobilization in response to phosphate starvation by directly facilitating the transcription of genes encoding S-like ribonucleases in Arabidopsis and tomato, respectively 
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520 |a Phosphorus (P) is a crucial element for plant growth and development, with inorganic phosphate (Pi) being the primary form absorbed by plant roots. However, soil often experiences Pi deficiency, leading to early leaf senescence and hastening the P re-mobilization from senescent leaves to young tissues. The PHOSPHATE STARVATION RESPONSE (PHR) proteins are widely acknowledged as pivotal regulatory factors in response to low Pi, and they may be involved in the regulation of leaf senescence and P remobilization triggered by Pi deprivation. Nevertheless, the mechanisms underlying the regulation of these processes require further elucidation. In this study, it was observed that overexpression of AtPHR1 accelerates leaf senescence, facilitates P transfer, and upregulates the expression of S-like RNase-encoding genes such as RNS1, RNS2, and RNS5. Subsequent investigations utilizing yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), and transient transcription expression assay (TTEA) revealed direct binding of AtPHR1 to the P1BS motifs within the promoters of these genes, thereby activating their transcription. Similarly, the overexpression of tomato PHR protein SlPHL1 also increased the transcript levels of the S-like RNase-encoding genes including LE and LX. Moreover, experiments using Y1H, EMSA, and TTEA confirmed that SlPHL1 directly binds to the promoters of these two genes through P1BS motifs and subsequently activates their transcription. Additionally, introducing SlPHL1 into Arabidopsis led to an enhanced P turnover and RNA degradation, along with an increase in the expression of RNS1 and RNS2. Further investigations revealed that similar to AtPHR1, SlPHL1 is capable of directly activating the transcription of RNS1 and RNS2. Taken together, these findings suggest that AtPHR1 and SlPHL1 play a pivotal role in facilitating P remobilization by directly stimulating the transcription of S-like RNase-encoding genes. This mechanism appears to be conserved across Arabidopsis and tomato, highlighting the importance of PHRs in optimizing plant P utilization 
650 4 |a Journal Article 
650 4 |a Arabidopsis 
650 4 |a AtPHR1 
650 4 |a Phosphate starvation 
650 4 |a Phosphorus remobilization 
650 4 |a S-like RNase 
650 4 |a SlPHL1 
650 4 |a Solanum lycopersicum 
650 7 |a Phosphorus  |2 NLM 
650 7 |a 27YLU75U4W  |2 NLM 
650 7 |a Phosphates  |2 NLM 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Ribonucleases  |2 NLM 
650 7 |a EC 3.1.-  |2 NLM 
650 7 |a Transcription Factors  |2 NLM 
650 7 |a PHR1 protein, Arabidopsis  |2 NLM 
700 1 |a Huang, Shaoxuan  |e verfasserin  |4 aut 
700 1 |a Zhang, Duanmei  |e verfasserin  |4 aut 
700 1 |a Zhu, Lin  |e verfasserin  |4 aut 
700 1 |a Chen, Mingxue  |e verfasserin  |4 aut 
700 1 |a Liu, Yanan  |e verfasserin  |4 aut 
700 1 |a Zhang, Lang  |e verfasserin  |4 aut 
700 1 |a Yang, Jie  |e verfasserin  |4 aut 
700 1 |a Zhang, Xinyao  |e verfasserin  |4 aut 
700 1 |a Yu, Yang  |e verfasserin  |4 aut 
700 1 |a Li, Yanxia  |e verfasserin  |4 aut 
700 1 |a An, Wenhao  |e verfasserin  |4 aut 
700 1 |a Yang, Shuyue  |e verfasserin  |4 aut 
700 1 |a Zhang, Yongqiang  |e verfasserin  |4 aut 
700 1 |a Liu, Zhongjuan  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 221(2025) vom: 15. Apr., Seite 109608  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnas 
773 1 8 |g volume:221  |g year:2025  |g day:15  |g month:04  |g pages:109608 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2025.109608  |3 Volltext 
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