RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis

RNA-sequencing (RNA-seq) technologies have advanced exponentially in recent years, however, the application of RNA-seq to Mycobacterium tuberculosis remains limited. We present a wet-lab and computational protocol for RNA-seq based transcriptomics that was tested on 12 replicates each of 11 clinical...

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Veröffentlicht in:BioTechniques. - 1991. - 77(2025), 1 vom: 26. Jan., Seite 23-34
1. Verfasser: Hiebert, Morgan R (VerfasserIn)
Weitere Verfasser: Sharma, Meenu K, Go, Alwyn, Bonner, Christine, Laminman, Vanessa, Graham, Morag, Soualhine, Hafid
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2025
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Mycobacterium tuberculosis RNA extraction RNA-sequencing next-generation sequencing transcriptomics RNA, Bacterial Pyrazinamide 2KNI5N06TI