Enhancing nature's palette through the epigenetic breeding of flower color in chrysanthemum

Enhancing nature's palette through the epigenetic breeding of flower color in chrysanthemum

© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.

Détails bibliographiques
Publié dans:The New phytologist. - 1979. - 245(2025), 5 vom: 21. März, Seite 2117-2132
Auteur principal: Li, Xueqi (Auteur)
Autres auteurs: Bu, Fanqi, Zhang, Man, Li, Zhuozheng, Zhang, Yu, Chen, Haowen, Xue, Wanjie, Guo, Ronghua, Qi, Jingze, Kim, Cholmin, Kawabata, Saneyuki, Wang, Yu, Zhang, Qingzhu, Li, Yuhua, Zhang, Yang
Format: Article en ligne
Langue:English
Publié: 2025
Accès à la collection:The New phytologist
Sujets:Journal Article CRISPR‐dCas9 Chrysanthemum DNA methylation epigenetic breeding flower color Plant Proteins Anthocyanins
Description
Résumé:© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
Flower color is an important character of ornamental plants and one of the main target traits for variety innovation. We previously identified a CmMYB6 epigenetic allele that affects the flower color in chrysanthemum, and changes in flower color are caused by the DNA methylation level of this gene. However, it is still unknown which DNA methyltransferases are involved in modifying the DNA methylation levels of this gene. Here, we used dead Cas9 (dCas9) together with DNA methyltransferases that methylate cytosine residues in the CHH context to target the CmMYB6 promoter through transient and stable transformation methods. We found that CmDRM2a increased the DNA methylation level of the CmMYB6 promoter, the expression of CmMYB6 decreased and a lighter flower color resulted. By contrast, both CmDRM2b and CmCMT2 enhanced DNA methylation levels of the CmMYB6 promoter, the expression of CmMYB6 increased and a deeper flower color resulted. Furthermore, the regulatory mechanism of DNA methyltransferase in the formation of chrysanthemum flower color was investigated, pointing to a new strategy for silencing or activating CmMYB6 epiallele to regulate anthocyanin synthesis. This lays a solid foundation for regulating flower color in chrysanthemum through epigenetic breeding
Description:Date Completed 29.04.2025
Date Revised 29.04.2025
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.20347