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|a 10.1093/jxb/erae414
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|a eng
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|a Rathore, Ray Singh
|e verfasserin
|4 aut
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|a Grain Lysine enrichment and improved stress tolerance in rice through protein engineering
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|c 2024
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|a Text
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|a ƒaComputermedien
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|a Date Revised 11.10.2024
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a © The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.
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|a Amino acids are a major source of nourishment for people living in regions where rice is a staple food. However, rice grain is deficient in essential amino acids, such as lysine. The activity of dihydrodipicolinate synthase (DHDPS) enzyme is crucial for lysine production in higher plants, but it is highly regulated through a feedback inhibition by its end product lysine, leading to its limited activity in the grain and resulting in low lysine accumulation. We identified lysine binding sites in the DHDPS enzyme and introduced key mutations to make it lysine feedback insensitive. Using in vivo analysis and functional complementation assays, we confirmed that protein engineering of the DHDPS renders it insensitive to lysine. Expression of mutated DHDPS resulted in 29 % higher lysine and 15 % higher protein accumulation in rice grains than the wild type. Importantly, the lysine content in transgenic grains was maintained in cooked rice. Further, the transgenic plants exhibited enhanced stress tolerance along with better antioxidant levels, improved photosynthesis, and higher grain yield compared to wild type plants. We have shown for the first time in rice that protein engineering of DHDPS can lead to accumulation of lysine in grains and impart abiotic stress tolerance. This approach could improve health in regions with nutrient deficiencies and environmental stressors that challenge food production and human health
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|a Journal Article
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|a - DHDPS
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|a Drought
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|a Grain yield
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|a Lysine
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|a Mutation
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|a Rice
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|a Salinity
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|a Mishra, Manjari
|e verfasserin
|4 aut
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|a Pareek, Ashwani
|e verfasserin
|4 aut
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|a Singla-Pareek, Sneh Lata
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
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|u http://dx.doi.org/10.1093/jxb/erae414
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