Dissecting the molecular basis of the ultra-large grain formation in rice

Dissecting the molecular basis of the ultra-large grain formation in rice

© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 243(2024), 6 vom: 07. Sept., Seite 2251-2264
1. Verfasser: Li, Fengcheng (VerfasserIn)
Weitere Verfasser: Wu, Lian, Li, Xiang, Chai, Yanan, Ruan, Nan, Wang, Ye, Xu, Na, Yu, Zhiwen, Wang, Xiaoche, Chen, Hao, Lu, Jiahao, Xu, Hai, Xu, Zhengjin, Chen, Wenfu, Xu, Quan
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article de novo assembly gene pyramiding grain shape rice variety improvement Plant Proteins
Beschreibung
Zusammenfassung:© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
The shape of rice grains not only determines the thousand-grain weight but also correlates closely with the grain quality. Here we identified an ultra-large grain accession (ULG) with a thousand-grain weight exceeding 60 g. The integrated analysis of QTL, BSA, de novo genome assembled, transcription sequencing, and gene editing was conducted to dissect the molecular basis of the ULG formation. The ULG pyramided advantageous alleles from at least four known grain-shaping genes, OsLG3, OsMADS1, GS3, GL3.1, and one novel locus, qULG2-b, which encoded a leucine-rich repeat receptor-like kinase. The collective impacts of OsLG3, OsMADS1, GS3, and GL3.1 on grain size were confirmed in transgenic plants and near-isogenic lines. The transcriptome analysis identified 112 genes cooperatively regulated by these four genes that were prominently involved in photosynthesis and carbon metabolism. By leveraging the pleiotropy of these genes, we enhanced the grain yield, appearance, and stress tolerance of rice var. SN265. Beyond showcasing the pyramiding of multiple grain size regulation genes that can produce ULG, our study provides a theoretical framework and valuable genomic resources for improving rice variety by leveraging the pleiotropy of grain size regulated genes
Beschreibung:Date Completed 22.08.2024
Date Revised 22.08.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.20001