Hop stunt viroid infection induces heterochromatin reorganization
© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
| Publié dans: | The New phytologist. - 1979. - 243(2024), 6 vom: 16. Sept., Seite 2351-2367 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2024
|
| Accès à la collection: | The New phytologist |
| Sujets: | Journal Article biotic stress defense epigenetics heterochromatin histone marks hop stunt viroid repressive epigenetic marks viroids Heterochromatin plus... |
| Résumé: | © 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation. Viroids are pathogenic noncoding RNAs that completely rely on their host molecular machinery to accomplish their life cycle. Several interactions between viroids and their host molecular machinery have been identified, including interference with epigenetic mechanisms such as DNA methylation. Despite this, whether viroids influence changes in other epigenetic marks such as histone modifications remained unknown. Epigenetic regulation is particularly important during pathogenesis processes because it might be a key regulator of the dynamism of the defense response. Here we have analyzed the changes taking place in Cucumis sativus (cucumber) facultative and constitutive heterochromatin during hop stunt viroid (HSVd) infection using chromatin immunoprecipitation (ChIP) of the two main heterochromatic marks: H3K9me2 and H3K27me3. We find that HSVd infection is associated with changes in both H3K27me3 and H3K9me2, with a tendency to decrease the levels of repressive epigenetic marks through infection progression. These epigenetic changes are connected to the transcriptional regulation of their expected targets, genes, and transposable elements. Indeed, several genes related to the defense response are targets of both epigenetic marks. Our results highlight another host regulatory mechanism affected by viroid infection, providing further information about the complexity of the multiple layers of interactions between pathogens/viroids and hosts/plants |
|---|---|
| Description: | Date Completed 22.08.2024 Date Revised 22.08.2024 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.19986 |